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. 2017 May 31;7:2554. doi: 10.1038/s41598-017-02818-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo imaging of CXCR4 expressing human melanoma lung metastasis by PepR-NIR750. Mice bearing PES43-derived lung metastases were i.v. injected with Pep-R-NIR750 and imaged 3 weeks after cells inoculation. Total body imaging studies were carried out 1 h–5 h–24 h–48 h after tracer injection with FMT 4000. The NIR image shows a strong signal of PepR-NIR750 in the lungs after 24 hours from injection.