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. 2017 May 31;7:2502. doi: 10.1038/s41598-017-02768-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mer is required for stability of adherens junction between CCs. (A–C) Apical tip of a wild-type (A and B) and Mer ^ts1 mutant (C) testis stained for E-cadherin (Blue), Vasa (Green), and Add (Red). GSCs are indicated by white dots. Hub (*). Bar, 10 µm. (D and E) Apical tip of wild-type (D) and Mer ^ts1 mutant (E) testis visualized for Arm-GFP (Green), Vasa (Blue), and Add (Red). (F and G) Transmission electron microscopy showing junctions between two somatic cells in a wild-type (F) and Mer ^ts1 (G) testis. Arrows indicate cell-cell junctions. Blue, double-headed arrows in (F) indicate gaps in cell-cell junction structure. We noted that Mer ^ts1 mutant somatic cells have numerous multilamellar bodies (yellow arrowhead), although its meaning is currently unclear. Bar, 500 nm.