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C2C12 differentiation induces mitochondrial biogenesis. A: MitoTracker green fluorescent images captured at ×20 magnification of C2C12 myoblasts vs. myotubes on days (D) 0 and 4 of differentiation. Representative Western blots (B) and graphical quantifications of COX-I (C), COX-IV (D), and Tfam (E) at days 0 and day 4 of differentiation. Data are represented as means ± SE and are measured in arbitrary units (AU). *P < 0.05 vs. day 0; n = 3 experiments.
