Fig. 7.

CHOP influences mitochondrial content basally and is not required for CCA-induced mitochondrial biogenesis. Cells were transfected with either 30 nM of scrambled (Scr) or CHOP siRNA (si-CHOP) on days 4 and 7 of differentiation. Myotubes underwent 4 days of successive chronic contractile activity (CCA) starting on day 5. A: CHOP representative blot and graphical representation. B: representative blots and graphical representations of mitochondrial markers COX-I (C), COX-IV (D), and Tfam (E). Data are expressed as means ± SE and are measured in arbitrary units (AU). πP < 0.05 vs. control (CON); *P < 0.05 main effect of CCA; φP < 0.05 main effect of CHOP siRNA; n = 7 to 11 experiments.