Fig. 4.
LCHF diet feeding does not affect mitochondrial biogenesis and lowers ATP production in skeletal muscle. A: gene expression in gastrocnemius muscle relative to 18S of genes involved in mitochondrial homeostasis (n = 6–8). B: protein levels of different mitochondrial chain complexes in gastrocnemius muscle relative to eukaryotic elongation factor 2 (eEF2) and representative immunoblots (n = 6). ATP5A, ATP synthase 5 alpha; NDUFB8, mitochondrial NADH dehydrogenase 1 beta subcomplex subunit 8. C and D: complex I-induced V̇o2 rate (OCR; C) and estimated ATP production (D) of isolated mitochondria from quadriceps muscle (n = 4–6). E and F: complex II-induced OCR (E) and estimated ATP production (F) of isolated mitochondria from quadriceps muscle (n = 4–6). Error bars represent SE. *Significant differences between genotypes: chow-fed CTRL and mKO mice and LCHF diet-fed CTRL and mKO mice (P < 0.05), respectively; #significant differences between conditions: chow and LCHF diet-fed CTRL and chow and LCHF diet-fed mKO mice (P < 0.05), respectively.