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. 2017 Feb 2;122(5):1095–1105. doi: 10.1152/japplphysiol.00862.2016

Fig. 3.

Fig. 3.

Myocardial apoptotic protein expression. A: representative immunostainings of Bcl-2 and Bax protein in the cytoplasm of the left ventricular myocytes isolated after 3 h of reperfusion. CON, control; RPre, remote liver ischemia preconditioning; RPost, remote liver ischemia postconditioning; RPre+RPost, the combination of remote liver ischemia preconditioning with remote liver ischemic postconditioning. Densitometric analysis of Bcl-2 (B), Bax (C), and Bcl-2/Bax (D) protein expression in rat left ventricles post-I/R is shown. Values are means ± SE; n = 4–6 per group. PEI, positive expressive index. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. sham; #P < 0.05 and ##P < 0.01 vs. CON; †P < 0.05 vs. RPre. Representative Western blots (top) and quantification (bottom) of cleaved caspase-3 (E) and caspase-3 (F) protein band density (normalized to GAPDH) in sham, CON, and liver-conditioned rat left ventricles are shown. Values are means ± SE; n = 4–5 per group. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. sham; ##P < 0.01 and ###P < 0.001 vs. CON.