Fig. 3.

Effect of hindlimb unloading (HU) on muscle protein synthesis in adult and old rats. Protein synthesis was measured in the soleus (A), medial gastrocnemius (MG; B), and tibialis anterior (TA; C) muscles of adult (9 mo, □) and old (29 mo, ■) rats after 3, 7, and 14 days of HU. Total protein, determined by stain-free imaging of the PVDF membrane, was used to normalize protein expression. Puromycin values are expressed as a percentage of each age-matched control group (n = 5–7/group). Values are means ± SE. *P < 0.05 vs. adult control. #P < 0.05 vs. old control. ^ϕP < 0.05 vs. adult at same time point.