Fig. 8.

Expression changes in FOXO1 and FOXO3a during hindlimb unloading (HU) in adult and old rats. mRNA expression of FOXO1 and FOXO3a was assessed by quantitative PCR in the soleus (A), medial gastrocnemius (MG; B), and tibialis anterior (TA; C) muscles of adult (9 mo, □) and old (29 mo, ■) rats after 3, 7, and 14 days of HU. Gene expression was normalized to tissue weight. Data are expressed as a fold change relative to the adult control group (n = 5–6/group). Values are means ± SE. *P < 0.05 vs. adult control. ^#P < 0.05 vs. old control. ^ϕP < 0.05 vs. adult at same time point.