Figure 1.

Modifications under the surface of detection. Improved sequencing methods have led to the discovery of millions of modification sites in all classes of RNAs. However, efficient detection of modifications is mostly possible at sites that undergo deamination. Given that more than 130 types of modified ribonucleotides are known to date, it can be expected that novel technologies will lead to a huge increase in detectable RNA modifications that are currently hidden underneath the (detection) surface.