Human ECFC-CM reduces infiltration of inflammatory cells in kidneys following I/R. Kidney resident monocytes were isolated from kidneys harvested 2 days postsurgery/treatment. A: gating strategy for FACS analysis. Lymphocytes were gated based on forward and side scatter plot. Total number of infiltrating monocytes (B), total number of CD4+ T cells (C), CD8+ T cells (D), total IL-17+ T cells (E), CD4+IL-17+ T cells (F), and IFN-γ+ cells (G) were quantified using fluorescence-activated cell sorting. Data are expressed as total number of each cell type per gram of tissue and are means ± SE. *P < 0.05 sham vs. I/R+vehicle by Student’s t-test. ϕP < 0.05 sham vs. I/R+ECFC-CM. #P < 0.05 I/R+vehicle vs. I/R+ ECFC-CM.