Fig. 5.

Uptake of ¹²⁵I-angiotensinogen is dependent on the outer mitochondrial membrane. A and B: purified sheep cortical (A) and HK-2 (B) mitochondria were exposed to the nonionic detergent digitonin (5 mg/ml) for various times to remove the outer mitochondrial membrane. Following the digitonin treatment, mitochondrial preparations were subjected to 10% SDS gel fractionation and immunoblotting with an antibody to VDAC. Digitonin treatment depleted the outer membrane marker VDAC in both sheep and human HK-2 mitochondria. C: digitonin treatment at 15 min significantly reduced ¹²⁵I-angiotensinogen uptake. Data are means ± SE; *P < 0.05 vs. control; n = 3.