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. 2017 Feb 17;312(5):H1096–H1104. doi: 10.1152/ajpheart.00680.2016

Fig. 4.

Fig. 4.

Role of cellular and mitochondrial-derived ROS in the formation of ceramide and PAI-1 eEVs. The number of eEVs (fold change) is significantly increased following treatment with C2 ceramide (15 µM), PAI-1 (20 ng/ml), and antimycin A (10 µM) compared with vehicle-treated control (n = 4 for treatment groups, n = 6 for control). PBA (10 μM) decreased only antimycin A-generated eEV formation (n = 3) compared with antimycin A alone, whereas mito PBA (10 μM) significantly decreased eEV formation compared with all three respective treatments (n = 3 all groups). Black bars represent no antioxidant treatment. *P < 0.05 vs. vehicle; †P < 0.05 vs. treatment.