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. 2017 Feb 17;312(5):H874–H885. doi: 10.1152/ajpheart.00637.2016

Fig. 5.

Fig. 5.

Epigenetic regulator DNA methyltransferase (DNMT)3a and proinflammatory IL-17A are increased and anti-inflammatory IL-10 is downregulated in the hypertensive kidney. A: protein (100 µg) was resolved on SDS-PAGE gels and probed with the appropriate antibodies overnight. Band intensities were quantified using ImageJ software. The bar graphs indicate mean intensities ± SD. n = 5 mice/group. *P < 0.05, control vs. ANG II; †P < 0.05, ANG II vs. ANG II + GYY. B: DNMT3a and IL-17 expression were increased in glomeruli and tubular areas of the kidney. Immunostaining for DNMT3a and IL-17A showed increased expression in glomeruli and proximal tubules, supporting Western blot data (white arrows). Magnification: ×60. Scale bars = 20 µm.