Table 1.

Primers used for methylation-specific PCR

Gene	Forward	Reverse
CBS, methylated	5′-AGTTTATTTAGATTCGGACGCG-3′	5′-GACCCAAAATTAACGAATATCGAC-3′
CBS, unmethylated	5′-GGAGTTTATTTAGATTTGGATGTGG-3′	5′-CAACCCAAAATTAACAAATATCAAC-3′
CSE, methylated	5′-AACGAAATGGGTAGTATTACGGTAC-3′	5′-ACCAACGATAATTAATTACGTCGAC-3′
CSE, unmethylated	5′-TGAAATGGGTAGTATTATGGTATGA-3′	5′-AACCAACAATAATTAATTACATCAAC-3′

CBS, cystathionine β-synthase; CSE, cystathionine γ-lyase.