Fig. 3.

Inflammatory profile analysis over time course of ALI. Lung histological evaluation of hematoxylin and eosin (A) and Masson’s trichrome (B) stainings of murine lung sections from vehicle- or LPS-treated (96 h) WT (Tie2.Cre⁻) and EC Cav-1^−/− (Tie2.Cre⁺) mice. Magnification: ×400. Scale bar: 400 µm. C: BALF total cell counts. D: lung MPO activity (U·min⁻¹·g⁻¹ wet lung) in tissue and BALF (E). F: quantification of collagen content of lung sections revealed by Masson’s trichrome stain. Images were quantified by using ImageJ software (% area/field; 5 fields by slide). Protein concentration (G), IL-6 (H), IL-6R (I), TGF-β1 (J), and TNF-α (K) were measured in cell-free BALF or lungs (L: IL-1β; M: IL-6) after 96 h of LPS challenge in WT and EC Cav-1^−/−; *P < 0.01, **P < 0.001, and ***P < 0.0001 indicate difference between vehicle and LPS-treated WT or EC Cav-1^−/−; #P < 0.01, ##P < 0.001, and ###P < 0.0001 indicate difference between both LPS-treated WT and EC Cav-1^−/− miceby one-way ANOVA followed by post hoc Newman-Keuls test (n = 3 animals/group).