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Effect of macrolides ± cigarette smoke extract (CSE) on NLRP3 in THP-1 macrophages. Azithromycin (Az), GS-459755 (G4), and GS-560660 (G5) were used at a concentration of 0.5 µg/ml and NLRP3 measured using immunofluorescence/confocal microscopy. Serial images (n = 10 per well) were captured from 5 experiments. *P < 0.05 vs. control (no CSE); †P < 0.05 vs. CSE unless otherwise indicated. Data analyzed using Friedman and Wilcoxon signed-rank tests.
