Table 1.
Gene list and qRT-PCR results from freshly isolated nasal epithelia and cultured bronchial epithelia
| Gene | Fresh | Cultured | Protein |
|---|---|---|---|
| SLC2A1 | 26.0 ± 0.21 | 22.2 ± 0.38 | Solute carrier family 2 (facilitated glucose transporter), member 1; glucose transporter 1 |
| SLC2A2 | ND | ND | Solute carrier family 2 (facilitated glucose transporter), member 2; glucose transporter 2 |
| SLC2A3 | 28.5 ± 0.61 | 32.7 ± 1.95 | Solute carrier family 3 (facilitated glucose transporter), member 3; glucose transporter 3 |
| SLC2A4 | 34.2 ± 0.74 | 30.1 ± 0.48 | Solute carrier family 4 (facilitated glucose transporter), member 4; glucose transporter 4 |
| SLC2A5 | 31.5 ± 0.20 | 32.2 ± 1.93 | Solute carrier family 5 (facilitated glucose transporter), member 5; glucose transporter 5 |
| SLC2A6 | 33.6 ± 0.51 | 29.3 ± 0.51 | Solute carrier family 6 (facilitated glucose transporter), member 6; glucose transporter 6 |
| SLC2A7 | ND | ND | Solute carrier family 7 (facilitated glucose transporter), member 7; glucose transporter 7 |
| SLC2A8 | 30.6 ± 0.11 | 27.8 ± 0.70 | Solute carrier family 8 (facilitated glucose transporter), member 8; glucose transporter 8 |
| SLC2A9 | 27.4 ± 0.15 | 26.2 ± 0.35 | Solute carrier family 9 (facilitated glucose transporter), member 9; glucose transporter 9 |
| SLC2A10 | 27.7 ± 0.14 | 27.0 ± 0.64 | Solute carrier family 10 (facilitated glucose transporter), member 10; glucose transporter 10 |
| SLC2A11 | 27.8 ± 0.04 | 27.5 ± 0.09 | Solute carrier family 11 (facilitated glucose transporter), member 11; glucose transporter 11 |
| SLC2A12 | 26.7 ± 0.16 | 27.1 ± 0.50 | Solute carrier family 12 (facilitated glucose transporter), member 12; glucose transporter 12 |
| SLC2A13 | 26.7 ± 0.19 | 27.5 ± 0.50 | Solute carrier family 13 (facilitated glucose transporter), member 13; proton (H+) myoinositol symporter |
| SLC5A1 | 28.2 ± 0.46 | 28.3 ± 0.40 | Solute carrier family 5 (sodium/glucose cotransporter), member 1; sodium-glucose transporter 1 |
| SLC5A2 | ND | ND | Solute carrier family 5 (sodium/glucose cotransporter), member 2; sodium-glucose transporter 2 |
| SLC5A4 | ND | ND | Solute carrier family 5 (glucose-activated ion channel), member 4; sodium-glucose transporter 3 |
| CFTR | 28.6 ± 0.22 | 27.4 ± 0.23 | Cystic fibrosis transmembrane conductance regulator |
| INSR | 26.7 ± 0.25 | 24.3 ± 0.94 | Insulin receptor |
| ACTB | 21.0 ± 0.27 | 19.0 ± 0.72 | Actin, β |
| HPRT1 | 26.0 ± 0.19 | 24.8 ± 0.25 | Hypoxanthine guanine phosphoribosyltransferase 1 |
| GAPDH | 18.5 ± 0.17 | 17.5 ± 0.56 | Glyceraldehyde-3-phosphate dehydrogenase |
Cycle threshold (Ct) data are presented as means ± SE. The abbreviated dataset from qRT-PCR results is presented in Fig. 3. An autoadjusted cycle threshold value of ≥35 is considered not detected (ND). Fresh, freshly scraped nasal epithelial cells from non-cystic fibrosis (CF) donors; Cultured, non-CF human bronchial epithelial primary cells grown at the air-liquid interface for a minimum of 4 wk.