Fig. 7.
Determination of upstream signals regulating nicotine-dependent reduced p38-MAPK phosphorylation. A: chemical inhibition of PKC-α does not reduce p38-MAPK phosphorylation. A representative blot is shown. B: quantitation of results in A, n = 8. C: nicotine-mediated reduction of p38-MAPK phosphorylation is blocked by rottlerin. D: quantitation of results in C, n = 8. *P < 0.05 vs. control/vehicle group. #P < 0.05 from both vehicle and nicotine control groups; 2-way ANOVA, Tukey’s test. Any samples in cropped images were run on the same gel with groups irrelevant to the study removed.