Fig. 1.
Generation of SPC-rtTA/tetO-Cre/FAKfl/fl mice (SC FAK) mice. A: alveolar epithelial cell (AEC)-specific deletion of focal adhesion kinase (FAK) is achieved by in triple transgenic SC FAK mice carrying the surfactant protein-C promoter-reverse tetracycline transactivator (SPC-rtTA) and CMV promoter-tetO-cre recombinase (CMV-tetO-Cre) transgenes crossed into floxed FAK background. Doxycycline chow was started postnatally at 1 mo and continued for 2 wk. B: immunoblot confirming that AECs from SC FAK mice have much less FAK expression compared with littermate control mouse lacking 1 of the 3 transgenes. Fibroblasts from SC FAK mice have similar levels of FAK compared with control mice. C and D: uninjured hematoxylin-eosin-stained lung sections (×200) of littermate control (C) and SC FAK mice (D).