Fig. 2.

Mice with lung epithelial cell-specific deletion of FAK have greater cellular apoptosis after bleomycin. A: 5 days after intrapulmonary saline or bleomycin injury, whole lung lysate was analyzed for phospho-FAK (p-FAK) and total FAK by immunoblot. B–D: 5 days after intrapulmonary bleomycin injury, cellular apoptosis was assessed by lung sections from littermate control mice (B) and SC FAK mice (C) that were stained with terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) (×200) and TUNEL-positive cells per field were quantified (D). P < 0.05; n = 5. E: 5 days after bleomycin or saline, whole lung active caspase-3/7 was measured in SC FAK and littermate control mice; n = 5–9. *P < 0.01, compared with bleomycin control mice.