Figure 3.

Administration of 2-deoxy-d-glucose (2-DG) attenuates sepsis-induced accumulation of immune cells and prevents intercellular cell adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) expression in the myocardium. A, Eight hours after cecal ligation and puncture (CLP)–induced sepsis, hearts were harvested and sectioned for immunostaining of immune cells with specific antibody NIMP-R1411b, which can recognize Ly-6G and Ly-6C neutrophils, monocytes, and macrophages. 2-DG prevents sepsis-induced increases in the expression of ICAM-1 (B) and VCAM-1 (C). n = 4–6 per group. 2-DG treatment attenuated lipopolysaccharide (LPS)–induced ICAM-1 (D) and VCAM-1 (E) expression in endothelial cells. Human umbilical vein endothelial cells were treated with LPS (1 µg/mL) in the presence or absence of 2-DG. The cells were harvested 12 hours after treatment. Cellular proteins were prepared for immunoblotting. n = 8 replicates in each group. *P < .05, **P < .01, and ***P < .001 compared with indicated groups.