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. 2017 Mar 22;215(9):1396–1406. doi: 10.1093/infdis/jix138

Figure 5.

Figure 5.

Administration of 2-deoxy-d-glucose (2-DG) attenuates sepsis-induced myocardial apoptosis. A, Hearts were harvested 8 hours after cecal ligation and puncture (CLP) sepsis and sectioned for TUNEL assay. B, Myocardial caspase-3/7 activity was analyzed by enzyme-linked immunosorbent assay. 2-DG treatment prevents sepsis-induced Bak (C) and Bax (D) expression in the myocardium. 2-DG treatment prevents lipopolysaccharide (LPS)–induced Bak (E) and Bax (F) expression in endothelial cells. n = 8 replicates/group. 2-DG treatment prevents sepsis-induced JNK phosphorylation in the myocardium (G; n = 4–6/group) and LPS-increased JNK phosphorylation in the endothelial cells (H). Human umbilical vein endothelial cells were treated with lipopolysaccharide (1 µg/mL) in the presence or absence of 2-DG. The cells were harvested 12 hours after treatment. Cellular proteins were prepared for immunoblotting. n = 8 replicates/group. *P < .05, **P < .01, and ***P < .001 compared with indicated groups.