Figure 5. Effect of rod inner segment conductances on the temporal characteristics of the photovoltage.

Normalized flash families are shown for the photocurrent (solid black), photovoltage (dashed red), and the photovoltage measured with a Cs⁺ internal solution (dotted blue) in the dark‐adapted state (top row) and following 70% bleaching of the visual pigment (bottom row). Normalized dim‐flash responses for photocurrent (solid black) and photovoltage (dashed red) under normal conditions and with Cs⁺ (dotted blue) are superimposed for the dark‐adapted state (top right) and following 70% bleaching (bottom right). Note that the speeding of the photovoltage with respect to the photocurrent is abolished with the Cs⁺ internal solution, and that this temporal filtering is less prominent at 70% bleach compared to the dark‐adapted state. The time‐to‐peak of the dim flash responses for the photocurrent, photovoltage and the photovoltage measured with a Cs⁺ internal solution were 300 ± 30 ms (n = 7), 300 ± 26 ms (n = 8) and 180 ± 9 ms (n = 8) for the dark‐adapted state, and 110 ± 10 ms (n = 4), 120 ± 5 ms (n = 6) and 80 ± 6 ms (n = 5) following the 70% bleach, respectively. The difference between the photovoltage and the photovoltage measured with a Cs⁺ internal solution was significantly different in each case (P < 0.001, Student's t test). [Color figure can be viewed at wileyonlinelibrary.com]