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. 2017 Mar 23;595(11):3591–3605. doi: 10.1113/JP273712

Figure 8. Prolactin activates pSTAT5 in the paraventricular nucleus of virgin and lactating rats.

Figure 8

A–D, representative images of pSTAT5 (nuclear staining) and oxytocin immunostaining (cytoplasmic staining) in the paraventricular nucleus of virgin and lactating rats after three injections of bromocriptine over 28–4 h before the experiment. E, mean (±SEM) proportion of oxytocin neurones expressing pSTAT5 in the paraventricular nucleus of virgin and lactating rats given either i.c.v. vehicle (n = 5 and 4, respectively) or 500 ng prolactin (n = 7 and 6, respectively) after three injections of bromocriptine over 28–4 h before the experiment. Two‐way ANOVA: main effect of reproductive status: F 1,21 = 0.27, P = 0.61; main effect of prolactin: F 1,21 = 118.78, P < 0.001; interaction: F 1,21 = 0.27, P = 0.61. F, mean (±SEM) proportion of pSTAT5‐positive neurones expressing oxytocin in the paraventricular nucleus of virgin and lactating rats given either i.c.v. vehicle (n = 5 and 4, respectively) or 500 ng prolactin (n = 7 and 6, respectively) after three injections of bromocriptine over 28–4 h before the experiment. Two‐way ANOVA: main effect of reproductive status: F 1,21 = 2.28, P = 0.60; main effect of prolactin: F 1,21 = 155.90, P < 0.001; interaction: F 1,21 = 2.28, P = 0.60. 3V: 3rd ventricle. [Color figure can be viewed at wileyonlinelibrary.com]