Table 2.

ERK1, pERK1, ERK2, pERK2, Akt, pAkt and β‐tubulin expression in the supraoptic nucleus

	ERK1	pERK1	ERK2	pERK2	Akt	pAkt	β‐Tubulin
Dioestrus + vehicle	33 582 ± 3886	67 504 ± 12 294	82 527 ± 11 135	1 36 186 ± 26 810	13 183 ± 1502	24 783 ± 2395	97 857 ± 14 998
	(n = 7)	(n = 7)	(n = 7)	(n = 7)	(n = 6)	(n = 6)	(n = 7)
Lactating + vehicle	28 492 ± 3886	82 094 ± 12 294	76 328 ± 11 135	1 58 925 ± 26 810	12 018 ± 1502	24 433 ± 2395	84 761 ± 14 998
	(n = 7)	(n = 7)	(n = 7)	(n = 7)	(n = 6)	(n = 6)	(n = 7)
Dioestrus + prolactin	29 140 ± 3635	73 092 ± 11 500	73 859 ± 10 416	1 40 454 ± 25 078	11 715 ± 1301	22 288 ± 2074	82 157 ± 14 029
	(n = 8)	(n = 8)	(n = 8)	(n = 8)	(n = 8)	(n = 8)	(n = 8)
Lactating + prolactin	29 477 ± 3635	60 502 ± 11 500	71 451 ± 10 416	1 08 821 ± 25 078	10 607 ± 1502	18 603 ± 2395	93 664 ± 14 029
	(n = 8)	(n = 8)	(n = 8)	(n = 8)	(n = 6)	(n = 6)	(n = 8)
Main effect of status	F 1,26 = 0.39	F 1,26 = 0.007	F 1,26 = 0.16	F 1,26 = 0.03	F 1,22 = 0.61	F 1,22 = 0.76	F 1,26 < 0.01
	P = 0.53	P = 0.93	P = 0.69	P = 0.87	P = 0.44	P = 0.39	P = 0.96
Main effect of prolactin	F 1,26 = 0.21	F 1,26 = 0.45	F 1,26 = 0.40	F 1,26 = 0.78	F 1,22 = 0.98	F 1,22 = 3.22	F 1,26 = 0.05
	P = 0.65	P = 0.51	P = 0.54	P = 0.38	P = 0.33	P = 0.09	P = 0.82
Interaction	F 1,26 = 0.52	F 1,26 = 1.30	F 1,26 = 0.03	F 1,26 = 1.10	F 1,22 < 0.01	F 1,22 = 0.52	F 1,26 = 0.72
	P = 0.48	P = 0.26	P = 0.86	P = 0.30	P = 0.98	P = 0.48	P = 0.40

Data shown are densitometry measurements (arbitrary units ± SEM) from Western blots for ERK1, pERK1, ERK2, pERK2, Akt, pAkt and β‐tubulin expression in the supraoptic nucleus after 2 μg i.c.v. prolactin.