Table 3.
ERK1, pERK1, ERK2, pERK2, Akt, pAkt and β‐tubulin expression in the paraventricular nucleus
| ERK1 | pERK1 | ERK2 | pERK2 | Akt | pAkt | β‐Tubulin | |
|---|---|---|---|---|---|---|---|
| Dioestrus + vehicle | 13 940 ± 1971 | 55 228 ± 17 841 | 46 986 ± 5604 | 1 81 329 ± 48 184 | 21 143 ± 2917 | 61 857 ± 8419 | 1 83 186 ± 37 256 |
| (n = 7) | (n = 7) | (n = 7) | (n = 7) | (n = 7) | (n = 7) | (n = 7) | |
| Lactating + vehicle | 14 108 ± 2129 | 84 183 ± 19 270 | 47 567 ± 6053 | 2 50 833 ± 52 045 | 18 667 ± 3151 | 71 683 ± 9094 | 1 57 300 ± 40 241 |
| (n = 6) | (n = 6) | (n = 6) | (n = 6) | (n = 6) | (n = 6) | (n = 6) | |
| Dioestrus + prolactin | 16 312 ± 1738 | 84 000 ± 15 734 | 47 044 ± 4943 | 2 25 489 ± 42 495 | 21 267 ± 2573 | 78 322 ± 7425 | 1 64 789 ± 32 857 |
| (n = 9) | (n = 9) | (n = 9) | (n = 9) | (n = 9) | (n = 9) | (n = 9) | |
| Lactating + prolactin | 14 863 ± 2129 | 63 917 ± 19 271 | 49 017 ± 6053 | 1 93 083 ± 52 045 | 26 333 ± 3151 | 80 467 ± 9094 | 1 30 217 ± 40 241 |
| (n = 6) | (n = 6) | (n = 6) | (n = 6) | (n = 6) | (n = 6) | (n = 6) | |
| Main effect of status | F 1,24 = 0.10 | F 1,24 = 0.06 | F 1,24 = 0.05 | F 1,24 = 0.14 | F 1,24 = 0.19 | F 1,24 = 0.49 | F 1,24 = 0.64 |
| P = 0.75 | P = 0.81 | P = 0.82 | P = 0.71 | P = 0.66 | P = 0.49 | P = 0.43 | |
| Main effect of prolactin | F 1,24 = 0.61 | F 1,24 = 0.06 | F 1,24 = 0.02 | F 1,24 = 0.02 | F 1,24 = 1.74 | F 1,24 = 2.19 | F 1,24 = 0.36 |
| P = 0.44 | P = 0.82 | P = 0.90 | P = 0.89 | P = 0.20 | P = 0.15 | P = 0.55 | |
| Interaction | F 1,24 = 0.16 | F 1,24 = 1.84 | F 1,24 = 0.01 | F 1,24 = 1.09 | F 1,24 = 1.63 | F 1,24 = 0.20 | F 1,24 = 0.01 |
| P = 0.69 | P = 0.19 | P = 0.90 | P = 0.31 | P = 0.21 | P = 0.66 | P = 0.91 |
Data shown are densitometry measurements (arbitrary units ± SEM) from Western blots for ERK1, pERK1, ERK2, pERK2, Akt, pAkt and β‐tubulin expression in the paraventricular nucleus after 2 μg i.c.v. prolactin.