Figure 5.

Osteo- and adipogenesis of iNCMSCs. The representative data for three lines is shown. (a) ALP staining at day 7 and calcium staining (alizarin red S and von Kossa staining) at day 14 of iNCMSCs cultured in osteogenic medium in 24-well plates. (b) Osteogenesis-related gene expression at days 3, 7, and 14. (c) Oil red staining of iNCMSCs cultured in adipogenesis medium at days 9 and 21. (d) Measurement of oil red positive droplets. (e) Adipogenesis-related gene expression at day 9, 15, and 21. Data are expressed as mean ± SD for three well replicate per group (b, d, e). Scale bars = 100 μm (a, c). Abbreviations: ALP: Alkaline phosphatase; G.M.: Growth medium.