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. 2017 Jun 1;7:46612. doi: 10.1038/srep46612

Figure 2. Neuronal stimulation of the contralesional cerebellar dentate nucleus (cLCN) activates pCREB expression.

Figure 2

(A) Diagram shows the location of implant site in the cLCN. (B) Representative image of cLCN implant location in the Thy1-ChR2-YFP mice. Positive expression of ChR2-YFP was observed in the cLCN (green) and GFAP immunostaining (red) illustrate fiber tract to the cLCN. Int = interposed nuclei, FN = fastigial nuclei. The area surrounding the LCN is the white matter. Scale Bar = 500 um. (C) Representative images of pCREB expression (red) and corresponding Thy1-ChR2-YFP expression (green) in the ipsilesional LCN (iLCN) and contralesional LCN (cLCN) in cLCN-stimulated and non-stimulated mice. Note that selective pCREB expression in the cLCN was observed in cLCN-stimulated mice at 90 minutes after stimulation, indicating that cLCN-stimulations led to neuronal activation in the cLCN. Scale bar = 250 um.