Figure EV4. Intrathecal naloxone antagonizes menthol‐ or morphine‐induced analgesia.

1. Quantification of the effect of intrathecal injection of naloxone on menthol‐induced analgesia in TRPM8^−/− mice. After control saline or naloxone was intrathecally injected in TRPM8^−/− mice, 15% menthol was applied to a hindpaw for a total of three times. After 10 min, 100 μM Cap was injected into the paw, and paw licking and lifting time was measured within 5 min.
2. Quantification of 100 μM Cap‐induced nociceptive responses in WT mice after intrathecal injection of morphine with or without naloxone.

Data information: The number of mice is indicated on top of each bar. Statistical significance was evaluated using two‐tailed t‐test. **P < 0.01; ***P < 0.001; the exact P‐values are indicated in Appendix Table S1. All the data are presented as the mean ± standard error of the mean (SEM).