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. 2017 Apr 10;9(6):802–815. doi: 10.15252/emmm.201607300

Figure 5. The effect of topical borneol in TRPM8−/− mice and WT mice with pharmacologically inhibited TRPM8.

Figure 5

  1. Quantification of the borneol effect on capsaicin (Cap)‐induced nociceptive responses in TRPM8−/− mice. The experimental procedure was the same as that in Fig 2A.
  2. Quantification of the borneol effect on formalin‐induced biphasic pain responses in TRPM8−/− mice. The experimental procedure was the same as that in Fig 2C.
  3. Quantification of the borneol effect on CFA‐induced mechanical hyperalgesia in TRPM8−/− mice. The experimental procedure was the same as that in Fig 2D. The statistical analysis was performed between the groups of Borneol (Ipsilateral) and Ethanol (Ipsilateral). n = 8 mice for each group.
  4. Quantification of the borneol effect on CFA‐induced thermal hyperalgesia in TRPM8−/− mice. The experimental procedure was the same as that in Fig 2E. The statistical analysis was performed between the groups of Borneol + CFA (n = 12) and Ethanol + CFA (n = 10). n = 8 for the group of Ethanol + Saline.
  5. Quantification of the borneol effect on Cap‐induced nociceptive responses in WT mice with TRPM8 being inhibited pharmacologically. TRPM8 antagonist AMTB was injected into a hindpaw 15 min before borneol application. Subsequent experimental procedure was the same as that in Fig 2A.
  6. Quantification of the borneol effect on formalin‐induced biphasic pain responses in WT mice with or without TRPM8 being inhibited pharmacologically. Saline or AMTB was injected into a hindpaw 15 min before borneol application. 15% borneol was applied to the paw for a total of three times, and 1.5% formalin was injected into the paw after 10 min. Paw licking and lifting time in both phases was measured. Saline was used as a control to AMTB and ethanol was used as a control to borneol.
Data information: In (A, B, E, F), the number of mice is indicated on top of each bar. Statistical significance was evaluated using two‐tailed t‐test. *P < 0.05; **P < 0.01; ***P < 0.001; ns indicates P > 0.05; the exact P‐values are indicated in Appendix Table S1. All the data are presented as the mean ± standard error of the mean (SEM).