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. 2017 Apr 10;18(6):1027–1037. doi: 10.15252/embr.201643764

Figure 3. VHH 1307 competes with P for binding to N.

Figure 3

  1. Magnified view and superposition of the C‐terminal domain of P (red) and VHH 1307 (gray) at their binding site at the N‐RNA C‐loop region.
  2. VHH binding to N and the N/P complex. Biotinylated, N‐specific VHHs, and a control VHH (αNP‐VHH1, influenza A nucleoprotein specific) were immobilized on streptavidin beads and N alone, or N in complex with P was added to the beads. Bound protein was eluted and subjected to 10% SDS–PAGE (75:1 acrylamide/bis‐acrylamide) and Coomassie staining. A representative gel from three independent experiments is shown.
  3. Band intensities of the immunoprecipitated N and coimmunoprecipitated P were quantified, and the relative amount of P compared to N is displayed. Average data from three independent experiments (± s.e.m.) are shown (n = 3; t‐test; *P < 0.05; **P < 0.01). Exact P‐values are shown in Table EV1.