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. 2017 Apr 18;36(11):1559–1576. doi: 10.15252/embj.201696392

Figure 3. Coordinated transcriptional upregulation of TCA cycle genes under nitrogen starvation is accompanied by upregulation of bZIP14 .

Figure 3

  • A, B
    Expression profile of TCA cycle genes (A) and bZIP14 co‐expression cluster (B) during nitrogen starvation. Cluster analysis was performed based on Fragments Per Kilobase Of Exon Per Million Fragments Mapped (FPKM) values normalized to the average FPKM value across all samples. Yellow and blue indicate gene induction and repression, respectively. Con, control cells; NoN, nitrogen‐depleted cells; NoP, phosphate‐starved cells; Noc, nocodazole‐treated cells; Dark, dark‐placed cells; H, hours.
  • C
    Expression pattern of TCA cycle genes during nitrogen starvation assessed by qRT–PCR on an independent repeat of the RNA‐Seq time course (n = 3). Cells grown in nitrogen‐repleted and ‐depleted conditions were harvested at the indicated time points to confirm the expression pattern of the TCA cycle genes observed in the RNA‐Seq analysis.
  • D
    Expression pattern of bZIP14 during nitrogen starvation assessed by qRT–PCR on an independent repeat of the RNA‐Seq time course. Values in the y‐axis represent the relative normalized expression ± SE of two biological repeats (each in three technical repeats) relative to the levels at time point 0 (set at 1). The x‐axis indicates time in hours.
Data information: AH: aconitate hydratase 2 (Phatr3_J26290); bHLH1a: basic helix‐loop‐helix1a (Phatr3_J44962); CS: citrate synthase (Phatr3_J30145); FBA3: fructose bisphosphate aldolase 3 (Phatr3_J29014); FUM: fumarase (Phatr3_J36139); FUM1: fumarase 1 (Phatr3_J19708); IsoCitDehy: isocitrate dehydrogenase (Phatr3_J14762); OGD: 2‐oxoglutarate dehydrogenase E1 component (Phatr3_J29016); MD: malate dehydrogenase (Phatr3_J42398); SCSa: succinate‐CoA ligase (Phatr3_J42015); SCSa2: succinyl‐CoA synthetase (Phatr3_J26921); SDH1: succinate dehydrogenase flavoprotein (Phatr3_J41812); SDH2: mitochondrial succinate dehydrogenase iron‐sulphur subunit (Phatr3_J52539); SDHcb: succinate dehydrogenase cb (Phatr3_J18516).