Table 2.
Oligonucleotides used for QuikChange mutagenesis
| No | Sequence motif | Primer sequence (5′→3′) |
|---|---|---|
| 1a | ZnF1 H>A mutation for | GCAAATATTATCTAAAAGTCGCCAACAGGAAACATACTGGGGAGAAACCC |
| 1b | ZnF1 H>A mutation rev | TCCCCAGTATGTTTCCTGTTGGCGACTTTTAGATAATATTTGCTGAGGAA |
| 2a | ZnF3 H>A mutation for | GAAGGATGGAGCTGCGGGTGGCCATGGTGTCTCACACAGGGGAGATGCCC |
| 2b | ZnF3 H>A mutation rev | TCCCCTGTGTGAGACACCATGGCCACCCGCAGCTCCATCCTTCGGCGGAA |
| 3a | ZnF4 H>A mutation for | AGAAGAAGGACTTGCAGAGCGCCATGATCAAACTTCATGGAGCCCCCAAG |
| 3b | ZnF4 H>A mutation rev | GCTCCATGAAGTTTGATCATGGCGCTCTGCAAGTCCTTCTTCTGCATGAA |
| 4a | ZnF5 H>A mutation for | CTCGGACAGAGCTGCAGCTGGCTGAAGCTTTCAAGCACCGTGGTGAGAAG |
| 4b | ZnF5 H>A mutation rev | CCACGGTGCTTGAAAGCTTCAGCCAGCTGCAGCTCTGTCCGAGACAGGAA |
| 5a | ZnF6 H>A mutation for | GCCGGAATGGCCTGCAGATGGCCATCAAGGCCAAGCACAGGAATGAGAGG |
| 5b | ZnF6 H>A mutation rev | TTCCTGTGCTTGGCCTTGATGGCCATCTGCAGGCCATTCCGGCTCGAGGC |
| 6a | ZnF7 H>A mutation for | AAAAGGCCAATCTCAACATGGCCCTGCGCACACACACGGGTGAGAAGCCC |
| 6b | ZnF7 H>A mutation rev | TCACCCGTGTGTGTGCGCAGGGCCATGTTGAGATTGGCCTTTTGGGTGAA |
| 7a | ZnF8 H>A mutation for | CCCAAGCCAGCCTGGACAAGGCCAACCGCACCCACACCGGGGAAAGGCCC |
| 7b | ZnF8 H>A mutation rev | TCCCCGGTGTGGGTGCGGTTGGCCTTGTCCAGGCTGGCTTGGGTTCGGAA |
| 8a | ZnF9 H>A mutation for | AGAAGGGGCCCCTCCTGAGGGCCGTGGCCAGCCGCCATCAGGAGGGCCGG |
| 8b | ZnF9 H>A mutation rev | TCCTGATGGCGGCTGGCCACGGCCCTCAGGAGGGGCCCCTTCTCAGTGAA |
| 9a | ZnF10 H>A mutation for | CCGTGGAGCAACTGCGTGTGGCCGTCAGACGGCACAAGGGGGTGAGGAAG |
| 9b | ZnF10 H>A mutation rev | ACCCCCTTGTGCCGTCTGACGGCCACACGCAGTTGCTCCACGGCTTTGAA |
| 10a | ZnF11 H>A mutation for | GACAGGCCCACCTGCGGAGGGCCATGGAGATCCACGACCGGGTAGAGAAC |
| 10b | ZnF11 H>A mutation rev | ACCCGGTCGTGGATCTCCATGGCCCTCCGCAGGTGGGCCTGTCGGGTAAA |
| 11a | ∆ZnF 1‐3 mutation for | GGGCATCTCCCCTGTCGGCACCGCTGTACCTTTTCTGTTC |
| 11b | ∆ZnF 1‐3 mutation rev | GGTACAGCGGTGCCGACAGGGGAGATGCCCTACAAGTGTT |
| 12a | ∆ZnF 4‐6 mutation for | TGGCCTCTCATTCCTGGCATCTCCCCTGTGTGAGACACCA |
| 12b | ∆ZnF 4‐6 mutation rev | CACAGGGGAGATGCCAGGAATGAGAGGCCACACGTATGTG |
| 13a | ∆ZnF 7‐9 mutation for | AGGAATGAGAGGCCACAGGAGGGCCGGCCCCACTTCTGCC |
| 13b | ∆ZnF 7‐9 mutation rev | GGGCCGGCCCTCCTGTGGCCTCTCATTCCTGTGCTTGGCC |
| 14a | ∆ZnF 7‐11 mutation for | AGGAATGAGAGGCCAGACCGGGTAGAGAACTACAACCCGC |
| 14b | ∆ZnF 7‐11 mutation rev | GTTCTCTACCCGGTCTGGCCTCTCATTCCTGTGCTTGGCC |
| 15a | ∆ZnF 1‐9 mutation for | GGTACAGCGGTGCCGCAGGAGGGCCGGCCCCACTTCTGCC |
| 15b | ∆ZnF 1‐9 mutation rev | GGGCCGGCCCTCCTGCGGCACCGCTGTACCTTTTCTGTTC |
| 16a | ∆ZnF 1‐10 mutation for | GGTACAGCGGTGCCGAAGGGGGTGAGGAAGTTTGAGTGCA |
| 16b | ∆ZnF 1‐10 mutation for | CTTCCTCACCCCCTTCGGCACCGCTGTACCTTTTCTGTTC |