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. 2017 Apr 18;18(6):962–981. doi: 10.15252/embr.201643519

Figure 2. Aβ1‐42 and Aβ3(pE)‐42 induce CREB shut‐off, drive Jacob in the nucleus and decrease nuclear pJacob levels.

Figure 2

Confocal images averaged from two sections of the nucleus of DIV18 primary hippocampal neurons stained for MAP2 and DAPI.
  • A, B
    (A) Neurons were co‐stained for pCREB (Ser133) and (B) the quantification revealed the decrease in nuclear pCREB immunoreactivity after treatment with Aβ1‐42 and Aβ3(pE)‐42.
  • C, D
    No change of total CREB levels was observed in nucleus of neurons co‐stained for pan‐CREB.
  • E–H
    There is an increase in nuclear Jacob immunoreactivity of neurons co‐stained with a pan‐Jacob antibody upon Aβ oligomers treatment (E, F) as well as a decrease in nuclear pJacob immunoreactivity (G, H). For the quantified channel (pCREB, CREB, pan‐Jacob and pJacob), original pixel intensities from 0 to 255 are represented as a gradient lookup table.
Data information: Scale bars, 10 μm. n corresponds to the number of nuclei from different neurons analysed from at least four independent coverslips and at least three independent cell cultures. P‐values versus control, by one‐way ANOVA. Data are represented as mean ± s.e.m.