Figure EV4. Co‐application of TNFR antagonist rescues Aβ3(pE)‐42‐caused decrease in pCREB .

1. Confocal images averaged from two sections of the nucleus of DIV18 primary hippocampal neurons stained for MAP2, DAPI and pCREB (Ser133) treated with Aβ1‐42 or Aβ3(pE)‐42 for 40 min with or without the TNFR antagonist. Original pixel intensities from 0 to 255 are represented as a gradient lookup table. Scale bar, 10 μm.
2. Only in case of Aβ3(pE)‐42 co‐application of the TNFR antagonist prevents CREB shut‐off. n corresponds to the number of nuclei from different neurons analysed from at least four independent coverslips from two independent cell cultures. P‐values versus control by one‐way ANOVA. Data are represented as mean ± s.e.m.