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. 2017 Apr 18;18(6):962–981. doi: 10.15252/embr.201643519

Figure 5. Aβ3(pE)‐42 does not bind prominently to neuronal membranes but is instead taken up efficiently by astrocytes.

Figure 5

  • A, B
    Confocal images of DIV18 neurons stained for (A) Aβ1‐42 or (B) Aβ3(pE)‐42 after 40 min of treatment demonstrate that Aβ1‐42 associates preferentially with neuronal membranes, whereas Aβ3(pE)‐42 is rather diffusely distributed in mixed neuronal culture. Scale bars, 10 μm (images of a whole neuron) and 5 μm (zoomed in panels).
  • C, D
    3D reconstructions (based on GFAP) of confocal images of astrocytes indicate that Aβ3(pE)‐42, but not Aβ1‐42, is taken up by astrocytes after 40 min of treatment.
  • E
    3D reconstruction of an astrocyte transfected with mGFP revealed that Aβ3(pE)‐42 localises within the cell 40 min following application.
  • F
    Co‐oligomerisation of Aβ1‐42 and Aβ3(pE)‐42 causes association of both species in one structure and leads to astrocytic uptake not only of Aβ3(pE)‐42 but also Aβ1‐42.