Figure 6. In AraC‐treated cultures Aβ3(pE)‐42 does not cause synapse loss, de‐phosphorylation of pCREB and accumulation of non‐phosphorylated Jacob in the nucleus.

• A
  Scheme representing the AraC treatment regime of neuronal cultures at DIV5.
• B
  Confocal images of representative dendrites, stained for Homer1, Synaptophysin and MAP2, demonstrating that the decrease in synaptic density in AraC‐treated cultures was evident only after Aβ1‐42 application. Scale bar, 5 μm.
• C
  Treatment with AraC selectively prevents the decrease in the mean synaptic density after Aβ3(pE)‐42 application. n corresponds to the number of separate dendritic segments on different neurons analysed from at least three independent coverslips and at least two independent cell cultures.
• D–K
  (D, E) In AraC‐treated cultures, only Aβ1‐42, but not Aβ3(pE)‐42, causes decrease in pCREB nuclear staining intensity with (F, G) unchanged CREB levels. Also (H, I) the pJacob nuclear levels and (J, K) Jacob accumulation significantly changes only in case of Aβ1‐42 treatment. Original pixel intensities from 0 to 255 are represented as a gradient lookup table. Scale bar, 10 μm.

Data information: n corresponds to the number of nuclei from different neurons analysed from at least four independent coverslips and at least three independent cell cultures. P‐values versus control, by one‐way ANOVA. Data are represented as mean ± s.e.m.