Figure 3. RANKL administration induces LTα upregulation specifically in thymic LTi cells after TBI .

• A
  Expression of RANK receptor in thymic LTi cells from UT WT (n = 6) mice and at d3 SL‐TBI (n = 6).
• B
  Expression level of LTα protein in thymic LTi cells from WT mice treated in vivo with PBS (n = 6), GST (n = 6), or RANKL‐GST (n = 6) during 3 days after SL‐TBI.
• C
  LTα protein was analyzed in thymocyte subsets and LTi cells from WT mice treated in vivo with GST (n = 9) or RANKL‐GST (n = 9) during 3 days after SL‐TBI. Results are represented as fold change relative to the GST condition. Data are pooled of three experiments.
• D
  Expression level of LTα protein in thymic LTi cells from WT mice treated in vivo with PBS (n = 6), an isotype control (Iso.) (n = 3), or a neutralizing anti‐RANKL antibody (IL22/5) (n = 6) during 3 days after SL‐TBI.
• E
  LTα protein was analyzed in thymocyte subsets and LTi cells from WT mice treated in vivo with an isotype control (n = 3), or a neutralizing anti‐RANKL antibody (n = 6) during 3 days after SL‐TBI. Results are represented as fold change relative to the isotype condition.
• F
  Expression of Ltα mRNA in the total thymus isolated from UT WT, Rorc^−/−, and ZAP‐70^−/− mice or at d3 SL‐TBI (n = 3–6 mice per genotype).
• G
  Expression of Ltα mRNA in the total thymus isolated from irradiated: WT, Rag2^−/− mice, and WT mice treated 3 days before with dexamethasone (Dexa). Data are pooled of two to three experiments (n = 6–12 mice per group).
• H
  Correlation of RANKL and LTα expression in thymic LTi cells during the course of BMT. pBMT: post‐bone marrow transplantation. Data are pooled of three independent experiments with similar results (n = 3–4 mice per group).
• I
  Expression level of LTα protein in thymic LTi cells from UT WT mice or at d3, d6, d10, and d21 after BMT. Data are pooled of three independent experiments with similar results (n = 3–4 mice per group).
• J
  Expression level of LTα protein analyzed by flow cytometry in thymic LTi cells from CD45.1 donor and CD45.2 host origin at d3 and d6 after BMT. Data are pooled of four experiments (n = 3–4 mice per group).
• K
  RANKL protein expression in LTi cells from WT and LTα^−/− mice at d3 SL‐TBI. Data are pooled of three experiments (n = 3–5 mice per group).
• L
  The expression of Ltα and Ltβ mRNAs was measured by qPCR in the total thymus from UT WT mice (n = 4) or at d3 SL‐TBI (n = 4).
• M
  MFI of LTα protein in CD45⁻ and CD45⁺ thymic cells from UT WT (n = 6) mice or at d3 SL‐TBI (n = 6).
• N, O
  Representative histogram of LTα (N) and LTβR‐Fc staining (O) in LTi cells from UT WT mice (n = 6) or at d3 SL‐TBI (n = 6) or L‐TBI (n = 6).

Data information: Data are shown as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001. Exact P‐values and statistical tests used to calculate them are provided in Appendix Table S2.