Figure EV3. LTα expression during BMT is required for peripheral T‐cell reconstitution.

• A–D
  Flow cytometry profiles and numbers of CD4⁺ and CD8⁺ T cells (A, C) as well as CD4⁺Foxp3⁺ Tregs (B, D) from CD45.1 donor origin in blood (A, B) and spleen (C, D) of WT CD45.1:WT and WT CD45.1:LTα^−/− mice at d21, d65, and d100 upon BMT. Significance relative to WT CD45.1:WT chimeras.
• E
  Histograms show numbers of CD62L⁺CD44⁻ naïve CD4⁺ and CD8⁺ T cells in the spleen of WT CD45.1:WT and WT CD45.1:LTα^−/− mice at d21 pBMT.
• F
  sjTREC were quantified by qPCR from genomic DNA of cell‐sorted splenic CD4⁺ and CD8⁺ T cells from WT CD45.1:WT and WT CD45.1:LTα^−/− mice at d21 pBMT.

Data information: Data are shown as mean ± SEM and are pooled of two independent experiments with similar results (n = 3–5 mice per group). *P < 0.05; **P < 0.01; one‐tailed Mann–Whitney U‐test. Exact P‐values are provided in Appendix Table S2.