Figure 4. LTα is critical for TEC regeneration during the course of BMT .

• A
  Expression level of LTβR protein in total TECs, cTECs, mTECs, and TEPC‐enriched cells from the thymus of UT WT mice (n = 6) and at d3 SL‐TBI (n = 6) was analyzed by flow cytometry. FMO: Fluorescence Minus One.
• B–F
  Flow cytometry profiles and numbers of total TECs (B); cTECs, mTECs (C); mTEC subsets (D); cTEC^hi, mTEC^hi, TEC^lo, mTEC^lo (E); and TEPC‐enriched cells (F) were analyzed in CD45^neg‐enriched cells by AutoMACS from the thymus of UT WT and LTα^−/− mice or in WT CD45.1:WT and WT CD45.1:LTα^−/− chimeras at d10, d21, and d65 upon BMT.
• G
  Numbers of total proliferating Ki‐67⁺ TECs, cTECs, mTECs, and TEPC‐enriched cells at the indicated time points.
• H
  The expression of mRNAs coding for Aire, Aire‐induced TRAs (Sp1 and Sp2); Aire‐independent TRA (casein β); Fezf2 and Fezf2‐induced TRAs (Apoc3, Fabp9, and Resp18) was measured by qPCR in CD45⁻ thymic stromal cells from WT CD45.1:WT and WT CD45.1:LTα^−/− mice at d65 after BMT. Significance relative to WT CD45.1:WT chimeras.

Data information: Data are shown as mean ± SEM and are pooled of three independent experiments with similar results (n = 3–5 mice per group). *P < 0.05; **P < 0.01. Exact P‐values and statistical tests used to calculate them are provided in Appendix Table S2.