Skip to main content
. 2017 Apr 5;18(6):947–961. doi: 10.15252/embr.201643147

Figure EV4. FKBP8 mutants that are not localized on mitochondria are unable to induce mitophagy.

Figure EV4

  1. HeLa cells were transiently transfected with mCherry‐GFP‐OMP25‐TM and 3×Flag‐LC3A together with Myc‐C1 as control and Myc‐FKBP8 WT, Myc‐FKBP8‐∆TM, or Myc‐FKBP8‐ER mutant expression constructs. The appearance of red‐only structures indicates acidified mitochondria. Scale bars, 10 μm.
  2. HeLa cells were transiently overexpressing mCherry‐GFP‐OMP25‐TM, 3×Flag‐LC3A, together with Myc‐tagged FKBP8 WT, FKBP8‐∆TM, or FKBP8‐ER. The graph bars represent the percentage of transfected cells containing red‐only dots indicative of mitophagy activity. Each bar shows the mean value with standard deviations from measurements on 50–70 cells obtained in three independent experiments.
  3. Western blot showing the expression levels of FKBP8 WT and mutant constructs, BNIP3, NIX, and the various ATG8 proteins in the cells used for experiments shown in Figs 5 and EV4B. Ponceau S staining is used as the loading control.