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. 2017 Mar 27;36(11):1543–1558. doi: 10.15252/embj.201694914

Figure 5. Thermoneutrality blunts the glycolytic rewiring in the BAT of HFD‐fed Mfn2‐adKO mice and sensitizes them to cold.

Figure 5

  1. High‐fat diet (HFD)‐fed control and Mfn2‐adKO littermates were housed for 8 weeks at thermoneutrality. Then, mice were sacrificed and protein homogenates were obtained from their BAT in order to test the indicated markers.
  2. Mice were housed as in (A). After 6 weeks at thermoneutrality, mice were fasted for 6 h and injected with insulin (1 U/kg). Then, blood glucose levels were measured at the indicated times.
  3. Mice were housed as in (A). After 7 weeks at thermoneutrality, mice were placed on a cold chamber at 6°C and body temperature was evaluated using a rectal thermometer at the indicated times. Mice were excluded from the test if their body temperature was ≤ 30°C.
Data information: All results are expressed as mean ± SEM of n = 10 (control; white circles; five males and five females) and n = 6 (Mfn2‐adKO; black circles; three males and three females) mice. * indicates statistically significant difference at P < 0.05 vs. respective control group (two‐tailed Student's t‐test).