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. 2017 Apr 4;18(6):894–905. doi: 10.15252/embr.201643564

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© 2017 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure EV3 — A–F
Predicted MWs of (A) Mis18α/β, (B) His‐GFP‐Mis18α/His‐Mis18β, (C) His‐Mis18α/His‐GFP‐Mis18β, (D) His‐GFP‐Mis18α_C‐term/His‐MBP‐Mis18β_C‐term, (E) Mis18α/β/Mis18BP1_20–130, (F) Mis18α/β/His‐Sumo‐Mis18BP1_20–130 complexes with different subunit compositions. These values together with the measured MWs from SEC‐MALS analysis shown in Fig 3 were used to deduce the correct subunit composition.