Figure 6. Inhibition of PIKfyve decreases cardiac oxidative stress and apoptosis induced by obesity.

1. Mitochondria‐derived ${\mathrm{O}}_2^{-}$ production was measured on heart cryosections using MitoSOX Red by confocal microscopy. Scale bar is 50 μm.
2. Quantification of MitoSOX fluorescence from (A).
3. TUNEL staining of heart cryosections showing apoptotic cells (arrows). The nuclei are stained in blue with DAPI. Scale bar is 50 μm.
4. Quantification from (C).
5. Bax expression level was measured by qRT‐PCR from heart tissues.

Data information: Data are presented as mean ± SEM. Student's t‐test, *P < 0.05; **P < 0.01 ***P < 0.001 between indicated conditions, n = 3–6 mice per group.