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. 2017 Apr 10;9(6):770–785. doi: 10.15252/emmm.201607096

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© 2017 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

A
Heart lysates from vehicle‐ or STA‐treated obese mice were immunoprecipitated using an anti‐SIRT3 antibody. Bound proteins were immunoblotted with an anti‐phospho‐serine antibody to reveal the phosphorylated SIRT3 (pSIRT3). As a loading control, total homogenates were probed with an anti‐SIRT3 antibody (SIRT3 total). Asterisk indicates antibody light chains. Quantification is shown on the right panel.

B, C
Same as in (A) but lysates were immunoprecipitated with an anti‐acetyl‐lysine antibody and immunoblotted for IDH2 (B) and SOD2 (C). Quantifications are shown on the right panels.

Data information: Data are presented as mean ± SEM. Student's t‐test, *P < 0.05; **P < 0.01, n = 3–8 mice per group. Source data are available online for this figure.