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. 2017 Jul;92(1):67–74. doi: 10.1124/mol.116.107995

Fig. 4.

Fig. 4.

A threshold negative charge of eight is required for maximal GC-A activity. 293 cells were transiently transfected with the indicated form of GC-A and membranes from these cells were assayed for GC activity for 5 minutes in the presence of 1 μM ANP, 1 mM ATP, and increasing concentrations of GTP. (A) Comparison between GC-A-8E, GC-A-7E (GC-A-8E with 513A), GC-A-6E (GC-A-7E with 510A), GC-A-5E (GC-A-6E with 506A), GC-A-4E (GC-A-5E with 502A), GC-A-3E (GC-A-4E with 500A), GC-A-2E (GC-A-3E with 497A), GC-A-1E (GC-A-2E with 487A), and GC-A-7A (WT-GC-A with 513A, 510A, 506A, 502A, 500A, 497A, 487S, and 473A) (n = 2 from two experiments). (B) Comparison between GC-A-8E, GC-A-8E-494E, and GC-A-8E-494E and 514E (n = 2 from two experiments). Vertical bars in the symbols represent the range of duplicate determinations.