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. 2017 Jul;92(1):67–74. doi: 10.1124/mol.116.107995

Fig. 6.

Fig. 6.

Differential effects of phosphorylation site mutations on phosphorylated WT-GC-A and GC-A-8E. 293 cells were transiently transfected with the indicated form of GC-A and assayed for GC activity for 5 minutes in the presence of 1 μM ANP, 1 mM ATP, and increasing concentrations of GTP. (A) Comparison between GC-A-8E-S497A and T500A as well as GC-A-WT-S497A and T500A (n = 2 from two experiments). Vertical bars in the symbols represent the range of duplicate determinations. (B) Comparison between WT-GC-A-S497A, WT-GC-A-S506A, WT-GC-A–T513A, GC-A-7E-S497A, GC-A-7E-S506A, and GC-A-7E-T513A (n = 2 from two experiments). Vertical bars in the symbols represent the range of duplicate determinations. (C) Pro-Q Diamond and Coomassie staining comparing phosphate and protein values for WT-GC-A, WT-GC-A-S497A, WT-GC-A-S506A, WT-GC-A–T513A, WT-GC-A–S497A and T513A, and WT-GC-A–7A. The ratio of the intensity of Pro-Q Diamond staining to Coomassie staining was normalized to WT (n = 4 from four independent immunoprecipitations). *P < 0.05 (significantly different from WT-GC-A). Vertical bars in the symbols represent the S.E.M.