Figure 4.

Average of FACS data from transfection efficiency of GFP-expressing NIH3T3 cells transfected with nTMag/GFP-DNA complexes in the absence of a magnetic field, in the presence of the nanoTherics static magnetic array and the nanoTherics magnefect-nano™ oscillating magnetic array (200 µm amplitude, f = 2 Hz) for 30 min, in comparison to lipid-based transfection (LF2000, for 30 min and 6 h). During transfection, cells were incubated at 37 °C and 5% CO₂. At 30 min post-transfection, the magnets were removed, and cells were incubated for 48 h before analysis. n = 6 for all samples.FACS: Fluorescence activated cell sorting; GFP: Green fluorescent protein; F: oscillation frequency.