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. 2017 Apr 28;9(5):441. doi: 10.3390/nu9050441

Figure 2.

Figure 2

Effect of PLA on tartrate resistant acid phosphatase (TRAP) activity and osteoclastogenesis. RAW264.7 murine macrophages were differentiated into osteoclasts in the presence of receptor activator of NF-κB ligand (RANKL) (15 ng/mL) and PLA (20–100 µM) for five days. (A) TRAP activity was determined as described in the methods. Results are shown as percentage of control and expressed as mean ± standard deviation; (B) Number of TRAP positive osteoclasts counted at different concentrations; (C) Effect of varying concentrations of PLA on osteoclast formation. TRAP positive (pink) osteoclasts are shown by yellow arrows. (Scale bar = 500 µm); (D) RAW264.7 murine macrophages were seeded onto glass coverslips with or without of PLA (100 µM) in combination with RANKL (15 ng/mL). Cells were stained for actin (red) and nuclei (blue) using Alexa fluor 568 phalloidin and Hoechst, respectively. (Scale bar = 100 µm). ** p < 0.01; *** p < 0.001; **** p < 0.0001 compared to control.