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. 2017 May 10;9(5):476. doi: 10.3390/nu9050476

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) T₁-weighted transversal image of the abdomen of a rat receiving a 13% (w/v) fructose solution. The positioning of a 5 × 2 × 4 mm³ voxel in the median lobe of the liver for ¹H-[¹³C] magnetic resonance spectroscopy (MRS) is indicated by the white square; (B) ¹H-[¹³C] MRS spectra from the voxel in panel A. Spectra were acquired after 5 days of [U-¹³C₆]glucose administration. Spectra without ¹³C editing, with ¹³C editing, and the calculated difference spectrum containing only ¹³C-coupled ¹H resonances (10× magnification) are shown. Peak annotations: Cho, choline; IHCL, intrahepatocellular lipids. Total IHCL content was quantified from the spectrum without ¹³C editing and ¹³C-enriched IHCL content was determined from the difference spectrum.